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pgem®-t easy 125 vector system ta cloning kit  (Promega)

 
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    Structured Review

    Promega pgem®-t easy 125 vector system ta cloning kit
    Pgem® T Easy 125 Vector System Ta Cloning Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ta+cloning+kit+(pgem-t+easy+vector+system/pgem++t+easy+125+vector+system+ta+cloning+kit/pm38246572-58-7-15
    Average 90 stars, based on 1 article reviews
    pgem®-t easy 125 vector system ta cloning kit - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Glycoprotein hormone subunit alpha 2 (GPHA2): A pituitary stem cell-expressed gene associated with NOTCH2 signaling.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Mos1-mediated insertional mutagenesis in Caenorhabditis elegans
    Article Snippet: The position of each oligonucleotide on the Mos1 sequence is shown in Figure 4 .Mos1 sequence: NCBI accession number X78906 (http:// www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db1⁄4nucleotide&val1⁄41335619) .dNTPs 10 mM (Fermentas, #R0182) .Taq DNA polymerase (Invitrogen, 10342-020) .T4 DNA ligase (Fermentas, #EL0015) .TA-cloning kit pGEM-T Easy Vector System I (Promega, A1360) .Transformation-competent Escherichia coli bacteria .QIAquick gel extraction kit (QIAgen, 28706) .QIAquick PCR purification kit (Qiagen, 28704) .DNEasy DNA extraction kit (Qiagen, 69504) .1 kb Plus DNA Ladder (Invitrogen, 10787-026) .Worm lysis solution (see REAGENT SETUP) .Restriction enzymes: Class A: HaeIII (R0108S), HpaII (R0171S) and Sau3AI (R0169S); class B: AluI (R0137S), HhaI (R0139S) and MseI (R0525S); NEB .Agarose gels for molecular biology .LB agar plates with appropriate antibiotic (according to TA-cloning kit instructions) .Nystatin solution (Sigma, N3503, http://www.sigmaaldrich.com; see REAGENT SETUP) .Nematode growth media (NGM) plates (see REAGENT SETUP) EQUIPMENT .Dedicated Pipetman to load PCR samples onto gels (Gilson) m CRITICAL This minimizes stock reagent contamination by Mos1 PCR products. p u or G g n i h si l b u P er u ta N 700 2 © n at u re p ro to co ls / m oc.er ut a n. w w w//: ptt h 1278 | VOL.2 NO.5 | 2007 | NATURE PROTOCOLS .Parafilm M* Laboratory Wrapping Film (Fisher, 13-374-10, http://www.fishersci.com) .Dissecting scope equipped with epifluorescence to visualize GFP in C. elegans .Heating waterbath .Thermal cycler (MJ Research PTC-200) .15, 20 and 25 1C incubators REAGENT SETUP C. elegans care and handling Instructions for maintaining C. elegans strains can be found at http://www.wormbook.org/chapters/www_strainmaintain/ strainmaintain.html.

    Article Title: Molecular Genealogy of a Mongol Queen’s Family and Her Possible Kinship with Genghis Khan
    Article Snippet: The aDNA treated with active or inactive UDG was cloned into the TA cloning vector provided in the pGEM-T Easy Vector System I TA cloning kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions.

    Article Title: The pea aphid uses a version of the terminal system during oviparous, but not viviparous, development
    Article Snippet: Products of PCR were amplified from an Acyrthosiphon pisum (strain LSR1) mixed stage cDNA library and cloned into pGEM T-easy using the pGEM T-easy vector system kit (Promega, Madison, USA) or into pCR II-TOPO using the TOPO TA cloning kit (Life Technologies, Grand Island, USA).

    Article Title: Subcellular Arrangement of Molecules for 2-Arachidonoyl-Glycerol-Mediated Retrograde Signaling and Its Physiological Contribution to Synaptic Modulation in the Striatum
    Article Snippet: After the TA cloning using a pGEM-T Easy Vector System I kit (Promega, Madison, WI), cDNA fragments were sequenced and excised by Bam HI and Eco RI.

    Clone Assay:

    Article Title: Hepatitis B virus: predominance of genotype D in primitive tribes of the Andaman and Nicobar islands, India (1989-1999).
    Article Snippet: .. Three strains suspected to be mixtures of different serotypes on the basis of electrophoretograms were cloned using a TA cloning kit (pGEM-T Easy Vector System, Promega) and sequenced. ..

    TA Cloning:

    Article Title: Hepatitis B virus: predominance of genotype D in primitive tribes of the Andaman and Nicobar islands, India (1989-1999).
    Article Snippet: .. Three strains suspected to be mixtures of different serotypes on the basis of electrophoretograms were cloned using a TA cloning kit (pGEM-T Easy Vector System, Promega) and sequenced. ..



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